DNA sequencing III : dealing with difficult templates / edited by Jan Kieleczawa.
Tipo de material: TextoIdioma: Inglés Detalles de publicación: Sudbury, Mass. : Jones and Bartlett Publishers, c2009.Descripción: xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cmISBN:- 9780763742973
- 9780763742973
- DNA sequencing three
- DNA sequencing 3
- 572.8/6 22
- QP624 .D17492 2009
- 2008 F-790
- QU 450
Tipo de ítem | Biblioteca actual | Signatura | Copia número | Estado | Fecha de vencimiento | Código de barras | Reserva de ítems | |
---|---|---|---|---|---|---|---|---|
Colección general | Biblioteca Yachay Tech | 572.86 D629 2009 (Navegar estantería(Abre debajo)) | Ej. 1 | Disponible | 002483 | |||
Colección general | Biblioteca Yachay Tech | 572.86 D629 2009 (Navegar estantería(Abre debajo)) | Ej. 2 | Disponible | 002484 | |||
Colección general | Biblioteca Yachay Tech | 572.86 D629 2009 (Navegar estantería(Abre debajo)) | Ej. 3 | Disponible | 002485 |
Includes bibliographical references and index.
Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.
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