Methods in yeast genetics and genomics : a Cold Spring Harbor Laboratory course manual / Maitreya J. Dunham, University of Washington, Marc R. Gartenberg, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Grant W. Brown, University of Toronto.
Tipo de material: TextoIdioma: Inglés Fecha de copyright: Cold Spring Harbor, New York : Cold Spring Harbor Laboratory Press, 2015Edición: 2015 edition / Maitreya J. Dunham, University of Washington, Marc R. Gartenberg, Robert Wood Johnson Medical School, Rutgers, State University of New Jersey, Grant W. Brown, University of TorontoDescripción: xvii, 233 pages : illustrations (some color) ; 26 cmISBN:- 9781621821342
- Methods in yeast genetics
- 579.562 23
- QK617.5 .K35 2015
Tipo de ítem | Biblioteca actual | Signatura | Copia número | Estado | Fecha de vencimiento | Código de barras | Reserva de ítems | |
---|---|---|---|---|---|---|---|---|
Colección general | Biblioteca Yachay Tech | 579.562 D917m 2015 (Navegar estantería(Abre debajo)) | Ej. 1 | Disponible | 005322 | |||
Colección general | Biblioteca Yachay Tech | 579.562 D917m 2015 (Navegar estantería(Abre debajo)) | Ej. 2 | Disponible | 005323 | |||
Colección general | Biblioteca Yachay Tech | 579.562 D917m 2015 (Navegar estantería(Abre debajo)) | Ej. 3 | Disponible | 005324 |
Includes index.
Includes bibliographical references.
Techniques and protocols -- Genomic Modifications with PCR Products -- High-Efficiency Yeast Transformation -- Using the C6 Cytometer -- Modified HoffmanDSWinston Genomic DNA Preparation -- Indirect Immunofluorescence Microscopy -- Yeast Vital Stains -- Actin Staining in Fixed Yeast Cells -- Preparation of Slides with Agarose Pads for Imaging of Live Yet Immobile Yeast -- Sporulation and Tetrad Dissection -- Making a Tetrad Dissection Needle -- EMS Mutagenesis -- Counting Yeast Cells with a Hemocytometer -- Flow Cytometry of Yeast DNA Content Using SYBR Green -- Storage and Handling of the Systematic Deletion Collection -- Scoring SGA Screens with SGATools -- Measuring DNA Concentration with the Qubit Fluorometer -- 17. Colony PCR
-- 18. Training for the Plate Race.
Methods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor Laboratory for the last 45 years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
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