Methods in yeast genetics and genomics :
Dunham, Maitreya J.
Methods in yeast genetics and genomics : a Cold Spring Harbor Laboratory course manual / Maitreya J. Dunham, University of Washington, Marc R. Gartenberg, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Grant W. Brown, University of Toronto. - 2015 edition / Maitreya J. Dunham, University of Washington, Marc R. Gartenberg, Robert Wood Johnson Medical School, Rutgers, State University of New Jersey, Grant W. Brown, University of Toronto. - xvii, 233 pages : illustrations (some color) ; 26 cm.
Includes index.
Includes bibliographical references.
Techniques and protocols -- Genomic Modifications with PCR Products -- High-Efficiency Yeast Transformation -- Using the C6 Cytometer -- Modified HoffmanDSWinston Genomic DNA Preparation -- Indirect Immunofluorescence Microscopy -- Yeast Vital Stains -- Actin Staining in Fixed Yeast Cells -- Preparation of Slides with Agarose Pads for Imaging of Live Yet Immobile Yeast -- Sporulation and Tetrad Dissection -- Making a Tetrad Dissection Needle -- EMS Mutagenesis -- Counting Yeast Cells with a Hemocytometer -- Flow Cytometry of Yeast DNA Content Using SYBR Green -- Storage and Handling of the Systematic Deletion Collection -- Scoring SGA Screens with SGATools -- Measuring DNA Concentration with the Qubit Fluorometer -- 17. Colony PCR
-- 18. Training for the Plate Race.
Methods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor Laboratory for the last 45 years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
9781621821342
2015017092
Yeast fungi--Genetics--Laboratory manuals.
Hongos de levadura--Genética--Manuales de laboratorio
QK617.5 / .K35 2015
579.562
Methods in yeast genetics and genomics : a Cold Spring Harbor Laboratory course manual / Maitreya J. Dunham, University of Washington, Marc R. Gartenberg, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Grant W. Brown, University of Toronto. - 2015 edition / Maitreya J. Dunham, University of Washington, Marc R. Gartenberg, Robert Wood Johnson Medical School, Rutgers, State University of New Jersey, Grant W. Brown, University of Toronto. - xvii, 233 pages : illustrations (some color) ; 26 cm.
Includes index.
Includes bibliographical references.
Techniques and protocols -- Genomic Modifications with PCR Products -- High-Efficiency Yeast Transformation -- Using the C6 Cytometer -- Modified HoffmanDSWinston Genomic DNA Preparation -- Indirect Immunofluorescence Microscopy -- Yeast Vital Stains -- Actin Staining in Fixed Yeast Cells -- Preparation of Slides with Agarose Pads for Imaging of Live Yet Immobile Yeast -- Sporulation and Tetrad Dissection -- Making a Tetrad Dissection Needle -- EMS Mutagenesis -- Counting Yeast Cells with a Hemocytometer -- Flow Cytometry of Yeast DNA Content Using SYBR Green -- Storage and Handling of the Systematic Deletion Collection -- Scoring SGA Screens with SGATools -- Measuring DNA Concentration with the Qubit Fluorometer -- 17. Colony PCR
-- 18. Training for the Plate Race.
Methods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor Laboratory for the last 45 years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
9781621821342
2015017092
Yeast fungi--Genetics--Laboratory manuals.
Hongos de levadura--Genética--Manuales de laboratorio
QK617.5 / .K35 2015
579.562