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020			_a0947946330
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040			_aEC-UrYT _cEC-UrYT
041			_aeng
082	0	4	_223 _a571.6381
245	0	0	_aAnimal cell culture : _ba practical approach / _cR I Freshney; series editor D. Rickwood and B. D. Hames.
250			_aFirst Edition
264	3	4	_aOxford, Washington D.C. : _bIRL Press ; _c1986.
300			_axiii, 248 pages : _billustrations ; _c23 cm.
490	0		_aPractical approach
500			_aIncludes appendix (pages 227-231) and index (pages 233-248).
504			_aIncludes reference.
505	2		_a1. Introduction: Principles of sterile technique and cell propagation -- 2. Towards chemically-defined, serum-free media for mammalian cell culture -- 3. Sealing-up of animal cell cultures -- 4. Preservation and characterisation -- 5. Separation of viable cells by centrifugal elutriation -- 6. Flow cytometry -- 7. Organ Culture -- 8. Cytotoxicity and viability assays -- 9. Autoradiographic localisation of specific cellular RNA sequences hybridised in situ to tritium-labelled probes.
650	2	4	_9143 _aCell culture
650	2	4	_96649 _aTissue culture
650	2	4	_98203 _aFlow cytometry
650	2	4	_911475 _aCytotoxicity
650	2	4	_96588 _aCultivo de células
650	2	4	_96650 _aCultivo de tejidos
650	2	4	_98204 _aCitometría de flujo
650	2	4	_911476 _aCitotoxicidad
700	1		_96648 _aFreshney, R. Ian, _eeditor
700	1		_911477 _aRickwood, D. _eseries editor
700	1		_911478 _aHames, B. D. _eseries editor
942			_2ddc _cLIBRO