000			02977nam a2200373 i 4500
001			Rebiun17781132
003			EC-UrYT
005			20210907142344.0
006			m o d
008			150531t20142014pl a | 000 0 eng d
020			_a9783110411324
040			_aCaPaEBR. _cCaPaEBR. _dEc-UrYT
041			_aeng
082	0	4	_a572.078 _223
100	1		_aGerczei, Timea _97014
245	1	0	_aBiochemistry laboratory manual for undergraduates: _ban inquiry-based approach / _cTimea Gerczei, Scott Pattison; managing editor, Anna Rulka
250			_aFirst Edition
264	3	4	_aWarsaw, [Poland] ; _aBerlin, [Germany] : _bDe Gruyter Open, _c2014
300			_a174 pages : _billustrations, (some color)
505	2		_aIntroducing the bacterial antibiotic sensor mini project -- Identifying concerved elements in the toxin sensor and designing mutants to test whether they are important for function -- Designing primers for site- directed mutahenesis -- Performing site-directed mutagenesis -- Purifying mutant toxin sensor DNA from baterial cells and evaluating its quality using agarose gel electrophoresis and UV spectroscopy -- Preparing DNA template for mutant RNA sensor synthesis using a restriction endocuclease -- Synthezing the jkkCD mutant toxin sensor RNA in vitro -- purifying the ykkCD mutant toxin sensor RNA and evaluating its purity using denaturing PAGE and UV spectrometry -- Evalating the ability of the jkkCD toxin sensor to recognize the antibiotic tetracycline using fluorescent quenching -- Evaluating antibiotic binding to blood serum albumin using fluorescence spectroscopy -- Understanding the importante of buffers in biological systems -- Molecular visualization of an enzyme, acetylcholinesterase -- Determining the efficiency of the enzyme acetycholine esterase using steady-state kinetic experiment -- Separation of the phosphatidycholines using reverse phase HPLC.
520	3		_aBiochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
650		0	_aBiochemistry _vLaboratory manuals _9326
650		0	_aBioquímica _vManuales de laboratorio _9130
650		0	_aMolecular biology _vLaboratory manuals _9341
650		0	_aBiología molecular _vManuales de laboratorio _9423
650		0	_aDrug resistance in microorganisms _vLaboratory manuals _97015
650		0	_aResistencia a los medicamentos en microorganismos _vManuales de laboratorio _97016
700	1		_aPattison, Robert _eautor _97017
942			_2ddc _cLIBRO
999			_c3001