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Biochemistry laboratory manual for undergraduates: an inquiry-based approach / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka

Por: Colaborador(es): Tipo de material: TextoTextoIdioma: Inglés Fecha de copyright: Warsaw, [Poland] ; Berlin, [Germany] : De Gruyter Open, 2014Edición: First EditionDescripción: 174 pages : illustrations, (some color)ISBN:
  • 9783110411324
Tema(s): Clasificación CDD:
  • 572.078  23
Contenidos parciales:
Introducing the bacterial antibiotic sensor mini project -- Identifying concerved elements in the toxin sensor and designing mutants to test whether they are important for function -- Designing primers for site- directed mutahenesis -- Performing site-directed mutagenesis -- Purifying mutant toxin sensor DNA from baterial cells and evaluating its quality using agarose gel electrophoresis and UV spectroscopy -- Preparing DNA template for mutant RNA sensor synthesis using a restriction endocuclease -- Synthezing the jkkCD mutant toxin sensor RNA in vitro -- purifying the ykkCD mutant toxin sensor RNA and evaluating its purity using denaturing PAGE and UV spectrometry -- Evalating the ability of the jkkCD toxin sensor to recognize the antibiotic tetracycline using fluorescent quenching -- Evaluating antibiotic binding to blood serum albumin using fluorescence spectroscopy -- Understanding the importante of buffers in biological systems -- Molecular visualization of an enzyme, acetylcholinesterase -- Determining the efficiency of the enzyme acetycholine esterase using steady-state kinetic experiment -- Separation of the phosphatidycholines using reverse phase HPLC.
Resumen: Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
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Tipo de ítem Biblioteca actual Signatura Copia número Estado Fecha de vencimiento Código de barras Reserva de ítems
Colección general Colección general Biblioteca Yachay Tech 572.078 G365b 2014 (Navegar estantería(Abre debajo)) Ej. 1 Disponible 005343
Colección general Colección general Biblioteca Yachay Tech 572.078 G365b 2014 (Navegar estantería(Abre debajo)) Ej. 2 Disponible 005344
Colección general Colección general Biblioteca Yachay Tech 572.078 G365b 2014 (Navegar estantería(Abre debajo)) Ej. 3 Disponible 005345
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Introducing the bacterial antibiotic sensor mini project -- Identifying concerved elements in the toxin sensor and designing mutants to test whether they are important for function -- Designing primers for site- directed mutahenesis -- Performing site-directed mutagenesis -- Purifying mutant toxin sensor DNA from baterial cells and evaluating its quality using agarose gel electrophoresis and UV spectroscopy -- Preparing DNA template for mutant RNA sensor synthesis using a restriction endocuclease -- Synthezing the jkkCD mutant toxin sensor RNA in vitro -- purifying the ykkCD mutant toxin sensor RNA and evaluating its purity using denaturing PAGE and UV spectrometry -- Evalating the ability of the jkkCD toxin sensor to recognize the antibiotic tetracycline using fluorescent quenching -- Evaluating antibiotic binding to blood serum albumin using fluorescence spectroscopy -- Understanding the importante of buffers in biological systems -- Molecular visualization of an enzyme, acetylcholinesterase -- Determining the efficiency of the enzyme acetycholine esterase using steady-state kinetic experiment -- Separation of the phosphatidycholines using reverse phase HPLC.

Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

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