In vitro culture of higher plants / by R.L.M. Pierik.

Originally published: Dordrecht ; Boston : M. Nijhoff ; Hingham, MA : Distributors for the U.S. and Canada, Kluwer, 1987.

Includes bibliographical references (p. 325-346) and index.

History -- Types of culture -- Laboratory equipment -- Preparation and composition of nutrient media -- Closure of test tubes and flasks -- Care of plant material -- Sterilization of plant material -- Isolation, inoculation and subculturing -- Mechanization -- The influence of plant material on growth and development -- The influence of physical factors on growth and development -- The transfer from nutrient medium to soil -- Aids to study -- Embryo culture -- Germination of orchid seeds -- Vegetative propagation of orchids -- Production of disease-free plants -- Vegetative propagation -- Somaclonal variation -- Test tube fertilization -- The production of haploids -- Genetic manipulation -- Miscellaneous applications -- In vitro cloning of plants in the Netherlands.

In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects.