Biochemistry laboratory manual for undergraduates: an inquiry-based approach / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka

Introducing the bacterial antibiotic sensor mini project -- Identifying concerved elements in the toxin sensor and designing mutants to test whether they are important for function -- Designing primers for site- directed mutahenesis -- Performing site-directed mutagenesis -- Purifying mutant toxin sensor DNA from baterial cells and evaluating its quality using agarose gel electrophoresis and UV spectroscopy -- Preparing DNA template for mutant RNA sensor synthesis using a restriction endocuclease -- Synthezing the jkkCD mutant toxin sensor RNA in vitro -- purifying the ykkCD mutant toxin sensor RNA and evaluating its purity using denaturing PAGE and UV spectrometry -- Evalating the ability of the jkkCD toxin sensor to recognize the antibiotic tetracycline using fluorescent quenching -- Evaluating antibiotic binding to blood serum albumin using fluorescence spectroscopy -- Understanding the importante of buffers in biological systems -- Molecular visualization of an enzyme, acetylcholinesterase -- Determining the efficiency of the enzyme acetycholine esterase using steady-state kinetic experiment -- Separation of the phosphatidycholines using reverse phase HPLC.

Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.