Biochemistry laboratory manual for undergraduates: an inquiry-based approach / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka

By: Gerczei, TimeaContributor(s): Pattison, Robert [autor]Material type: TextTextLanguage: English Copyright date: Warsaw, [Poland] ; Berlin, [Germany] : De Gruyter Open, 2014Edition: First EditionDescription: 174 pages : illustrations, (some color)ISBN: 9783110411324Subject(s): Biochemistry -- Laboratory manuals | Bioquímica -- Manuales de laboratorio | Molecular biology -- Laboratory manuals | Biología molecular -- Manuales de laboratorio | Drug resistance in microorganisms -- Laboratory manuals | Resistencia a los medicamentos en microorganismos -- Manuales de laboratorioDDC classification: 572.078
Partial contents:
Introducing the bacterial antibiotic sensor mini project -- Identifying concerved elements in the toxin sensor and designing mutants to test whether they are important for function -- Designing primers for site- directed mutahenesis -- Performing site-directed mutagenesis -- Purifying mutant toxin sensor DNA from baterial cells and evaluating its quality using agarose gel electrophoresis and UV spectroscopy -- Preparing DNA template for mutant RNA sensor synthesis using a restriction endocuclease -- Synthezing the jkkCD mutant toxin sensor RNA in vitro -- purifying the ykkCD mutant toxin sensor RNA and evaluating its purity using denaturing PAGE and UV spectrometry -- Evalating the ability of the jkkCD toxin sensor to recognize the antibiotic tetracycline using fluorescent quenching -- Evaluating antibiotic binding to blood serum albumin using fluorescence spectroscopy -- Understanding the importante of buffers in biological systems -- Molecular visualization of an enzyme, acetylcholinesterase -- Determining the efficiency of the enzyme acetycholine esterase using steady-state kinetic experiment -- Separation of the phosphatidycholines using reverse phase HPLC.
Abstract: Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
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Item type Current library Call number Copy number Status Date due Barcode Item holds
Libro académico Libro académico Biblioteca del Campus
572.078 G365b 2014 (Browse shelf (Opens below)) Ej. 1 Available 005343
Libro académico Libro académico Biblioteca del Campus
572.078 G365b 2014 (Browse shelf (Opens below)) Ej. 2 Available 005344
Libro académico Libro académico Biblioteca del Campus
572.078 G365b 2014 (Browse shelf (Opens below)) Ej. 3 Available 005345
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Introducing the bacterial antibiotic sensor mini project -- Identifying concerved elements in the toxin sensor and designing mutants to test whether they are important for function -- Designing primers for site- directed mutahenesis -- Performing site-directed mutagenesis -- Purifying mutant toxin sensor DNA from baterial cells and evaluating its quality using agarose gel electrophoresis and UV spectroscopy -- Preparing DNA template for mutant RNA sensor synthesis using a restriction endocuclease -- Synthezing the jkkCD mutant toxin sensor RNA in vitro -- purifying the ykkCD mutant toxin sensor RNA and evaluating its purity using denaturing PAGE and UV spectrometry -- Evalating the ability of the jkkCD toxin sensor to recognize the antibiotic tetracycline using fluorescent quenching -- Evaluating antibiotic binding to blood serum albumin using fluorescence spectroscopy -- Understanding the importante of buffers in biological systems -- Molecular visualization of an enzyme, acetylcholinesterase -- Determining the efficiency of the enzyme acetycholine esterase using steady-state kinetic experiment -- Separation of the phosphatidycholines using reverse phase HPLC.

Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

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