New antibiotic targets / edited by W. Scott Champney.Material type: TextLanguage: English Series: Methods in molecular medicine ; 142Copyright date: Totowa, N.J. : Humana Press , 2008Edition: First EditionDescription: xi, 274 pages : illustrations ; 24 cmISBN: 9781588299154; 1588299155; 9781597452465; 1597452467Subject(s): Drug resistance in microorganisms | Drug targeting | Medical virology | Medicine | Immunology | Resistencia a los medicamentos en microorganismos | Segmentación de drogas | Virología médica | Medicina | InmunologíaDDC classification: 616.9041
|Item type||Current library||Call number||Copy number||Status||Date due||Barcode||Item holds|
|Libro académico||Biblioteca del Campus||616.9041 N53212 2008 (Browse shelf (Opens below))||Ej. 1, Vol. 142||Available||000491|
|Libro académico||Biblioteca del Campus||616.9041 N53212 2008 (Browse shelf (Opens below))||Ej. 2, Vol. 142||Available||001051|
Includes bibliographical references.
1. Biocomputational Strategies for Microbial Drug Target Identification -- 2. Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities -- 3. A Method to Assay Inhibitors of DNA Polymerase III Activity -- 4. Methods to Identify and Characterize Inhibitors of Bacterial RNA Polymerase -- 5. Methods to Assay Inhibitors of tRNA Synthetase Activity -- 6. Three Methods to Assay Inhibitors of Ribosomal Subunit Assembly -- 7. Inhibition of Chaperone-dependent Bacterial Ribosome Biogenesis -- 8. Assays for the Identification of Inhibitors Targeting Specific Translational Steps -- 9. SPARK - A New Peptidyl Transferase Activity Assay -- 10. High-throughput Screening of Peptide Deformylase Inhibitors -- 11. A Method to Assay Penicillin Binding Proteins -- 12. A Method to Assay Inhibitors of Lipopolysaccharide Synthesis -- 13. Methods for Assessing the Structure and Function of Cationic Antimicrobial Peptides -- 14. Flow Cytometry of Bacterial Membrane Potential and Permeability -- 15. Bacterial Efflux Pump Inhibitors -- 16. Mycobacterium tuberculosis Beta-ketoacyl Acyl Carrier Protein Synthase III (mtFabH) Assay - Principle and Method -- 17. Screening for Compounds that Affect the Interaction between Bacterial Two- Component Signal Transduction Response Regulator Protein and Cognate Promoter DNA -- 18. The Activity of rRNA Resistance Methyltransferases Assessed by MALDI Mass Spectrometry -- 19. Assays for Beta-lactamase Activity and Inhibition -- 20. Studies of Enzymes that Cause Resistance to Aminoglycoside Antibiotics.
A crisis is developing in the treatment of infectious diseases, with microorganisms frequently showing resistance to currently prescribed antibiotics. Fewer and fewer antimicrobial agents are now available to treat these infections. In "New Antibiotic Targets, " W. Scott Champney examines specific techniques which can be used to explore new drug targets and the effectiveness of new antibiotics. By testing new antimicrobial agents and modified existing drugs, the most vulnerable cell processes, such as cell wall and membrane synthesis, DNA replication, RNA transcription and protein synthesis, can be better exploited. This in-depth volume, however, delves even deeper by identifying additional novel cellular targets for these new therapies. With detailed methods and protocols for the identification and assay of these targets, "New Antibiotic Targets" provides laboratory investigators with the vital tools they need to test the antimicrobial potential of products and to curb the rise of so many infectious diseases.