Techniques in molecular biology : Volume 2 /
Techniques in molecular biology : Volume 2 /
edited by John M. Walker and Wim Gaastra
- First edition
- iv, 332 pages : illustrations ; 24 cm.
Includes glossary (pages 320-325) and index (pages 326-332).
Preface -- 1. Micromethods in protein chemistry -- 2. Peptide synthesis -- 3. Affinity chromatography -- 4. The enzyme linked immunosorbent assay -- 5. Monoclonal antibodies -- 6. Plasmid derived cloning vectors -- 7. The Ti-plasmid of agrobacterium tumefaciens as a tool for genetic engineering in plants -- 8. Cloning in yeast -- 9. Cloning in bacteriophage lambda -- 10. The dot-blot technique -- 11. DNA-mediated gene transfer into mammalian cells -- 12. In vitro transcription -- 13. Chemical synthesis of oligodeoxyribonucleotides -- 14. Targeted mutagenesis of cloned DNA -- 15. DNA footprinting and related techniques for analysing protein-DNA interactions -- 16. Autoradiography and fluorography -- 17. Computer applications to studying DNA.
0709936737 0709936532
Molecular biology--Technique
Molecular biology
Molecular biology--Methodology
Genetic engineering--Methodology
Biología molecular--Técnica
Biología molecular
Biología molecular--Metodología
Ingeniería genética--Metodología
572.8
Includes glossary (pages 320-325) and index (pages 326-332).
Preface -- 1. Micromethods in protein chemistry -- 2. Peptide synthesis -- 3. Affinity chromatography -- 4. The enzyme linked immunosorbent assay -- 5. Monoclonal antibodies -- 6. Plasmid derived cloning vectors -- 7. The Ti-plasmid of agrobacterium tumefaciens as a tool for genetic engineering in plants -- 8. Cloning in yeast -- 9. Cloning in bacteriophage lambda -- 10. The dot-blot technique -- 11. DNA-mediated gene transfer into mammalian cells -- 12. In vitro transcription -- 13. Chemical synthesis of oligodeoxyribonucleotides -- 14. Targeted mutagenesis of cloned DNA -- 15. DNA footprinting and related techniques for analysing protein-DNA interactions -- 16. Autoradiography and fluorography -- 17. Computer applications to studying DNA.
0709936737 0709936532
Molecular biology--Technique
Molecular biology
Molecular biology--Methodology
Genetic engineering--Methodology
Biología molecular--Técnica
Biología molecular
Biología molecular--Metodología
Ingeniería genética--Metodología
572.8