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999			_c2987
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003			EC-UrYT
005			20200826125745.0
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008			150429s2015 nyua b 001 0 eng
010			_a 2015017092
020			_a9781621821342
040			_aEc-UrYT _bEc-UrYT _cEc-UrYT _erda _dEc-UrYT
041			_aeng
042			_apcc
050	0	0	_aQK617.5 _b.K35 2015
082	0	4	_a579.562 _223
100	1		_aDunham, Maitreya J. _96956
240	1	0	_aMethods in yeast genetics
245	1	0	_aMethods in yeast genetics and genomics : _ba Cold Spring Harbor Laboratory course manual / _cMaitreya J. Dunham, University of Washington, Marc R. Gartenberg, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Grant W. Brown, University of Toronto.
250			_a2015 edition / _bMaitreya J. Dunham, University of Washington, Marc R. Gartenberg, Robert Wood Johnson Medical School, Rutgers, State University of New Jersey, Grant W. Brown, University of Toronto.
264	3	4	_aCold Spring Harbor, New York : _bCold Spring Harbor Laboratory Press, _c2015.
300			_axvii, 233 pages : _billustrations (some color) ; _c26 cm.
500			_aIncludes index.
504			_aIncludes bibliographical references.
505	2		_aTechniques and protocols -- Genomic Modifications with PCR Products -- High-Efficiency Yeast Transformation -- Using the C6 Cytometer -- Modified HoffmanDSWinston Genomic DNA Preparation -- Indirect Immunofluorescence Microscopy -- Yeast Vital Stains -- Actin Staining in Fixed Yeast Cells -- Preparation of Slides with Agarose Pads for Imaging of Live Yet Immobile Yeast -- Sporulation and Tetrad Dissection -- Making a Tetrad Dissection Needle -- EMS Mutagenesis -- Counting Yeast Cells with a Hemocytometer -- Flow Cytometry of Yeast DNA Content Using SYBR Green -- Storage and Handling of the Systematic Deletion Collection -- Scoring SGA Screens with SGATools -- Measuring DNA Concentration with the Qubit Fluorometer -- 17. Colony PCR -- 18. Training for the Plate Race.
520	3		_aMethods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor Laboratory for the last 45 years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
650	2	4	_aYeast fungi _xGenetics _vLaboratory manuals. _96957
650	2	4	_aHongos de levadura _xGenética _vManuales de laboratorio _96958
700	1		_aGartenberg, Marc R. _96959 _eautor
700	1		_aBrown, Grant W. _96960 _eautor
942			_2ddc _cLIBRO