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Capillary electrophoresis of nucleic acids / edited by Keith R. Mitchelson and Jing Cheng. Volume 1; Introduction to the capillary electrophoresis of nucleic acids

Contributor(s): Mitchelson, Keith R [editor] | Cheng, Jing, 1963- [editor].
Material type: materialTypeLabelBookSeries: Methods in molecular biology v. 162-163.Copyright date: Totowa, N.J. : Humana Press, 2001Edition: First Edition.Description: xx, 484 pages illustrations ; 24 cm.ISBN: 9780896037793.Subject(s): Nucleic acids -- Separation | Capillary electrophoresis | Electroforesis capilarDDC classification: 572.8 Online resources: Publisher description | Table of contents only
Contents:
Vol. 1: Overview: The Application of Capillary Electrophoresis for DNA Polymorphism Analysis -- DNA Separation Mechanisms During Capillary Electrophoresis -- Purification Methods for Preparing Polymerase Chain Reaction Products for Capillary Electrophoresis Analysis -- Quantitative Measurements -- Microchip-Based Capillary Electrophoresis Systems -- DNA Sequencing by Capillary Array Electrophoresis -- Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophoresis -- Influence of Polymer Concentration and Polymer Composition on Capillary Electrophoresis of DNA -- Capillary Coatings: Choices for Capillary Electrophoresis of DNA -- Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis -- Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis -- Sieving Matrix Selection -- Parameters Affecting Capillary Electrophoretic Separation of DNA -- DNA Separation by Capillary Electrophoresis in Lyotropic Polymer Liquid Crystals -- Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material -- DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium -- DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries -- Capillary Electrophoresis with Glycerol as an Additive - Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels -- Robust Field Inversion Capillary Electrophoretic Separation of Long DNA Fragments -- Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments -- Collection of Capillary Electrophoresis Fractions on a Moving Membrane -- Separation of Supercoiled DNA Using Capillary Electrophoresis -- Quality Control of Nucleotides and Primers for PCR ---Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis -- Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides -- Capillary Electrophoresis Separation of Ribonucleosides -- On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Capillaries -- Capillary Electrophoresis–Mass Spectrometric Analysis of DNA Adducts -- Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis -- Assay for DNA Damage Using Immunochemical Recognition and Capillary Electrophoresis -- Analysis of DNA Damage Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry -- Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Sieving Electrophoresis -- Capillary Electrophoresis of Urinary Normal and Modified Nucleosides of Cancer Patients
Abstract: The advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has generated a host of DNA analytical procedures. In these novel and wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms
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Item type Current location Call number Copy number Status Date due Item holds
Libro académico Libro académico Biblioteca del Campus
572.8 C243 2001 (Browse shelf) Ej. 1 Available
Total holds: 0

Includes bibliographical references and index.

Vol. 1: Overview: The Application of Capillary Electrophoresis
for DNA Polymorphism Analysis -- DNA Separation Mechanisms During Capillary Electrophoresis -- Purification Methods for Preparing Polymerase Chain Reaction Products for Capillary Electrophoresis Analysis -- Quantitative Measurements -- Microchip-Based Capillary Electrophoresis Systems -- DNA Sequencing by Capillary Array Electrophoresis -- Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing
Fragments by Capillary Electrophoresis -- Influence of Polymer Concentration and Polymer Composition
on Capillary Electrophoresis of DNA -- Capillary Coatings: Choices for Capillary Electrophoresis of DNA -- Replaceable Polymers for DNA Sequencing by Capillary
Electrophoresis -- Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis -- Sieving Matrix Selection -- Parameters Affecting Capillary Electrophoretic Separation of DNA -- DNA Separation by Capillary Electrophoresis in Lyotropic Polymer Liquid Crystals -- Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material -- DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium -- DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries -- Capillary Electrophoresis with Glycerol as an Additive - Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels -- Robust Field Inversion Capillary Electrophoretic Separation
of Long DNA Fragments -- Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments -- Collection of Capillary Electrophoresis Fractions on a Moving Membrane -- Separation of Supercoiled DNA Using Capillary Electrophoresis -- Quality Control of Nucleotides and Primers for PCR ---Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis -- Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides -- Capillary Electrophoresis Separation of Ribonucleosides -- On-Line Preconcentration and Separation of Antisense
Oligonucleotides by ITP-CE in Dextran-Filled Capillaries -- Capillary Electrophoresis–Mass Spectrometric Analysis
of DNA Adducts -- Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis -- Assay for DNA Damage Using Immunochemical Recognition
and Capillary Electrophoresis -- Analysis of DNA Damage Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry -- Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Sieving Electrophoresis -- Capillary Electrophoresis of Urinary Normal
and Modified Nucleosides of Cancer Patients

The advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has generated a host of DNA analytical procedures. In these novel and wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms

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