Effect of the Electric field on the Biomineralization of Collagen / Fiorella Paulet Ortiz Mosquera ; tutor Antonio Díaz Barrios
Tipo de material:
TextoIdioma: Inglés Idioma del resumen: Español Fecha de copyright: Urcuquí, 2022Descripción: 94 hojas : ilustraciones (algunas a color) ; 30 cm + 1 CD-ROMTema(s): Recursos en línea: Nota de disertación: Trabajo de integración curricular (Químico/a). Universidad de Investigación de Tecnología Experimental Yachay. Urcuquí, 2022 Resumen: Collagen/hydroxyapatite hybrids are promising biomimetic materials that can replace or temporarily substitute bone tissues. The present work is focused on evaluating an electric field's effect on collagen biomineralization using a double-diffusion system. The methodological principle consisted in applying an electric field on the incubation medium to promote the opposite migration of ions into collagen membranes and produce hydroxyapatite (HA). Two physically separated solutions were used for the incubation medium, one rich in phosphate ions (solution A) and the other in calcium ions (solution B), and their effect was evaluated against the use of Simulated Body Fluid (SBF). Moreover, taking advantage of the polarizability properties of collagen, the benefits of pre-polarizing the organic membranes and the effect of incubation time were also assessed. Our results proved that the electric field, the membrane pre-polarization, and the ion's physical separation significantly accelerate the mineralization process of collagen. FTIR and Raman structure analysis confirmed the formation of HA after biomineralization and allowed to determine the mineralization degree of the synthesized samples quantitatively. It was found that the application of the electric field influenced the collagen structure and its interactions with the mineral phase. The increment of the mineralization degrees proved to enhance the photoluminescence properties of the collagen/HA materials. However, the electric properties, including the dielectric constant and conductivity, were reduced with the increment of the mineral deposition. These results might provide a useful approach for future applications in manufacturing biomimetic bone-like materials.
| Tipo de ítem | Biblioteca actual | Signatura | Copia número | Estado | Fecha de vencimiento | Código de barras | Reserva de ítems | |
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Tesis
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Biblioteca Yachay Tech | ECQI0132 (Navegar estantería(Abre debajo)) | 1 | No para préstamo | T000499 |
Trabajo de integración curricular (Químico/a). Universidad de Investigación de Tecnología Experimental Yachay. Urcuquí, 2022
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Trabajo de integración curricular con acceso abierto
Texto (Hypertexto links)
Collagen/hydroxyapatite hybrids are promising biomimetic materials that can replace or temporarily substitute bone tissues. The present work is focused on evaluating an electric field's effect on collagen biomineralization using a double-diffusion system. The methodological principle consisted in applying an electric field on the incubation medium to promote the opposite migration of ions into collagen membranes and produce hydroxyapatite (HA). Two physically separated solutions were used for the incubation medium, one rich in phosphate ions (solution A) and the other in calcium ions (solution B), and their effect was evaluated against the use of Simulated Body Fluid (SBF). Moreover, taking advantage of the polarizability properties of collagen, the benefits of pre-polarizing the organic membranes and the effect of incubation time were also assessed. Our results proved that the electric field, the membrane pre-polarization, and the ion's physical separation significantly accelerate the mineralization process of collagen. FTIR and Raman structure analysis confirmed the formation of HA after biomineralization and allowed to determine the mineralization degree of the synthesized samples quantitatively. It was found that the application of the electric field influenced the collagen structure and its interactions with the mineral phase. The increment of the mineralization degrees proved to enhance the photoluminescence properties of the collagen/HA materials. However, the electric properties, including the dielectric constant and conductivity, were reduced with the increment of the mineral deposition. These results might provide a useful approach for future applications in manufacturing biomimetic bone-like materials.
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