Includes index (pages 463-480).

Includes bibliographic reference.

Part 1 Image enhancement techniques -- 1. Video-Enhanced Microscopy -- 2. Computer- Aided Light Microscopy -- 3. Image Intensification of Stained, Functioning and Growing Neurons -- 4. Analysis of Molecular Distribution in Single Cells Using a Digital Imaging Microscope -- 5. Attempts to Synthesize a Red-Fluorescing Dye for Intracellular Injection -- Part 2 Optical probes of membrane potential -- 6. Optical Monitoring of Membrane Potential; Methods of Multisite Optical Measurement -- 7. Optical Studies of the Mechanism of Membrane Potential Sensitivity of Merocyanine 540 -- 8. Simultaneous Monitoring of Activity of Many Neurons from Invertebrate Ganglia Using a Multielement Detecting System -- 9. Optical Studies of Excitation ans Secretion at Vertebrate Nerve Terminals -- 10. Real-Time Optical Mapping of Neuronal -- 11. Optical Determination of Electrical Properties of Red Blood Cell and Ehrlich Ascites Tumor Cell Membranes with Fluorescent Dyes -- 12 An Acousto-Optically Steered Laser Scanning System for Measurement of Action Potential Spread in Intact Heart -- Part 3 Intracellular indicators -- 13. Practical Aspects for the Use of Photoproteins as Biological Calcium Indicators -- 14. Strategies for the Selective Measurement of Calcium in Various Regions of an Axon -- 15. Polychromator for Recording Optical Absorbance Changes from Single Cells -- 16. Calcium Transients in Frog Skeletal Muscle Fibers Injected with Azo1, a Tetracarboxylate Ca2+ Indicator -- 17. Intracellular pH of Limulus Ventral Photoreceptor Cells: Measurement with Phenol Red -- 18. Intracellularly Trapped pH Indicators -- 19. New Tetracarboxylate Chelators for Fluorescence Measurement and Photochemical Manipulation of Cytosolic Free Calcium Concentrations -- 20. The Use of Tetracarboxylate Fluorescent Indicators in the Measurement and Control of Intracellular Free Calcium Ions -- Part 4 Photobleaching and photoactivation -- 21. Membrane Dynamics Studied by Fluorescence Correlation Spectroscopy and Photobleaching Recovery -- 22. Caged ATP as a Tool in Active Transport Research -- 23. Desing and Application of Photolabile Intracellular Probes -- 25. Experiments with Photoisomerizable Molecules at Nicotinic Acetylcholine Receptors in Cells and Membrane Patches from Rat Muscle.