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Arabidopsis protocols / edited by Jose J. Sanchez-Serrano, Centro Nacional de Biotecnologia, CSIC, Madrid, Spain, Julio Salinas, Departmento Biologia de Plantas, Centro de Investigaciones Biologicas, CSIC, Madrid, Spain.

Contributor(s): Sánchez-Serrano, Jose J [editor] | Salinas, Julio [editor].
Material type: materialTypeLabelBookSeries: Methods in molecular biology ; 1062.Copyright date: New York : Humana Press, 2014Edition: Third edition.Description: xvi, 726 pages : illustrations (some color) ; 26 cm.ISBN: 9781627035798.Subject(s): Arabidopsis -- Laboratory manuals | Arabidopsis -- Molecular aspects -- Laboratory manualsDDC classification: 583.64 Online resources: Publisher description | Table of contents only
Partial contents:
Growing Arabidopsis: Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation and Genetic Crosses -- Using Arabidopsis Related Model Species (ARMS): Growth, Genetic Transformation and Comparative Genomics -- A. Bressan and Albino Maggio 3. Growing Arabidopsis in vitro: Cell Suspensions, in vitro Culture, and Regeneration -- Arabidopsis resources: Arabidopsis Database and Stock Resources -- Bioinformatic Tools in Arabidopsis Research -- Genetic techniques: Exploiting Natural Variation in Arabidopsis -- Agrobacterium tumefaciens Mediated Transient Transformation of Arabidopsis -- iTilling: Personalized Mutation -- Tailor Made Mutations in Arabidopsis Using Zinc-Finger Nucleases -- The Use of Artificial Micro RNA Technology to Control Gene Expression in Arabidopsis -- Generation and Identification of Arabidopsis EMS Mutants -- Generation and Identification of Arabidopsis T-DNA Insertion Mutants -- Identification of EMS-induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing -- Arabidopsis Transformation with Large Bacterial Artificial Chromosomes -- Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP) -- Molecular biological techniques: Next Generation Mapping of Genetic Mutations Using Bulk Population Sequencing -- Chemical Fingerprinting of Arabidopsis Using Fourier-transform Infrared (FT-IR) Spectroscopic Approaches -- A Pipeline for 15N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis -- Gene Expression Profiling Using DNA Microarrays -- Forward Chemical Genetic Screening -- Highly-Reproducible ChIP-On-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thaliana -- Cell biological techniques: Fluorescence Microscopy -- Immunocytochemical Fluorescent In Situ Visualization of Proteins in Arabidopsis -- High Pressure Freezing and Freeze Substitution of Arabidopsis For Electron Microscopy -- Applications of Fluorescent Marker Proteins in Plant Cell Biology -- Flow Cytometry.
Abstract: Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the "omics" tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches.
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Libro académico Libro académico Biblioteca del Campus
583.64 A6588 2014 (Browse shelf) Ej. 1 Available
Libro académico Libro académico Biblioteca del Campus
583.64 A6588 2014 (Browse shelf) Ej. 2 Available
Libro académico Libro académico Biblioteca del Campus
583.64 A6588 2014 (Browse shelf) Ej. 3 Available
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Growing Arabidopsis: Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation and Genetic Crosses -- Using Arabidopsis Related Model Species (ARMS): Growth, Genetic Transformation and Comparative Genomics -- A. Bressan and Albino Maggio 3. Growing Arabidopsis in vitro: Cell Suspensions, in vitro Culture, and Regeneration -- Arabidopsis resources: Arabidopsis Database and Stock Resources -- Bioinformatic Tools in Arabidopsis Research -- Genetic techniques: Exploiting Natural Variation in Arabidopsis -- Agrobacterium tumefaciens Mediated Transient Transformation of Arabidopsis -- iTilling: Personalized Mutation -- Tailor Made Mutations in Arabidopsis Using Zinc-Finger Nucleases -- The Use of Artificial Micro RNA Technology to Control Gene Expression in Arabidopsis -- Generation and Identification of Arabidopsis EMS Mutants -- Generation and Identification of Arabidopsis T-DNA Insertion Mutants -- Identification of EMS-induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing -- Arabidopsis Transformation with Large Bacterial Artificial Chromosomes -- Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP) -- Molecular biological techniques: Next Generation Mapping of Genetic Mutations Using Bulk Population Sequencing -- Chemical Fingerprinting of Arabidopsis Using Fourier-transform Infrared (FT-IR) Spectroscopic Approaches -- A Pipeline for 15N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis -- Gene Expression Profiling Using DNA Microarrays -- Forward Chemical Genetic Screening -- Highly-Reproducible ChIP-On-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thaliana -- Cell biological techniques: Fluorescence Microscopy -- Immunocytochemical Fluorescent In Situ Visualization of Proteins in Arabidopsis -- High Pressure Freezing and Freeze Substitution of Arabidopsis For Electron Microscopy -- Applications of Fluorescent Marker Proteins in Plant Cell Biology -- Flow Cytometry.

Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the "omics" tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches.

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